Corso di laurea magistrale in Chimica Clinica Forense e dello Sport

Inhibition of cytochrome P450-mediated drug metabolism by a concomitantly administered second drug is one of the major causes of drug–drug interactions in humans.

The availability of recombinant and chimeric forms of cytochrome P450 3A4 either in solution or immobilised allows for high-throughput testing of such interactions for evaluating new compounds as potential drugs and assess adverse drug-drug interactions.

The project will involve measurements of the inhibition of the turnover of a typical substrate of Cyt P450 3A4 by various compounds either in solution by a spectrophotometric assay or by electrochemically-driven catalysis of the enzyme immobilised on Au electrodes and subsequent quantification of products by HPLC.